Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

Mixture of Xylose and Glucose Affects Xylitol Production by Pichia guilliermondii: Model Prediction Using Artificial Neural Network

Production of several yeast products occur in presence of mixtures of monosaccharides. To study effect of xylose and glucose mixtures with system aeration and nitrogen source as the other two operative variables on xylitol production by Pichia guilliermondii, the present work was defined. Artificial Neural Network (ANN) strategy was used to athematically show interplay between these three c...

Obtaining partial purified xylose reductase from Candida guilliermondii

The enzymatic bioconversion of xylose into xylitol by xylose reductase (XR) is an alternative for chemical and microbiological processes. The partial purified XR was obtained by using the following three procedures: an agarose column, a membrane reactor or an Amicon Ultra-15 50K Centrifugal Filter device at yields of 40%, 7% and 67%, respectively.

Validation of the species Pichia guilliermondii.

Many species of yeasts exist in nature in haploid form. If strongly heterothallic, strains of such species produce no ascospores when tested individually. They do mate if sexually reactive strains of opposite sex are mixed on a sporulation medium. In 1952, L. J. Wickerham and K. A. Burton (J. Bacteriol. 63:449, 1952) reported that all strains of an imperfect form known variously as Dematium cho...

Efficient Production of L - Lactic Acid from Xylose by Pichia stipitis 1 2

Efficient production of L-lactic acid from xylose by Pichia stipitis.

Microbial conversion of renewable raw materials to useful products is an important objective in industrial biotechnology. Pichia stipitis, a yeast that naturally ferments xylose, was genetically engineered for l-(+)-lactate production. We constructed a P. stipitis strain that expressed the l-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentat...